Pro Tools | First Main Windows : Transport Window : Basic Transport Controls and Counters : Play
 
Play
Starts playback or (if the Record button was clicked first) recording from the Timeline insertion point or the Play Start Marker location if Dynamic Transport mode is enabled.
*You can also begin playback by pressing the Spacebar, or with the Numeric Keypad mode set to Transport, pressing 0.
Right-clicking the Play button lets you select the playback mode from a pop-up menu:
Normal
Loop
With the Transport stopped, Control-click Play (Mac) or Start-click Play (Windows) to toggle Loop Playback mode. When enabled, a loop symbol appears in the Play button and Pro Tools plays continuously from the beginning of the selection to the end.
*For more information on loop playback, see “Loop Playback Mode” on page 203.